Composite

Part:BBa_K1520519

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-23)

PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter

PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter. If gold ions exist, rfp will not be produced.

Suicidewithoutgold.jpg

this is part of our part BBa_K1520509 [1] and the picture is also part of BBa_K1520509, since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.

K1520509.jpg

From left to right, Marker(1Kb), PgolTS-golB-PgolB-tetR-Ter, PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter.

The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 548
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
biology
controlaTc, tetracyline
directionForward
functionRep
ligandstetracycline
negative_regulators1
o_h
o_l
positive_regulators
proteinTetR
swissproP04483
tagLVA